LOCUS       40924_31940       12205 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pMpGWB432 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T.
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha
  JOURNAL   PLoS ONE 10 (9), E0138876 (2015)
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12205)
  AUTHORS   Nishihama R, Ishizaki K, Kohchi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, 
            Graduate School of Biostudies; Kitashirakawa-oiwake-cho, Sakyo-ku, 
            Kyoto, Kyoto 606-8502, Japan URL :http://www.lif.kyoto-u.ac.jp/e/
REFERENCE   3  (bases 1 to 12205)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12205)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "9"; pages: "E0138876"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, Graduate 
            School of Biostudies"; volume: " Kitashirakawa-oiwake-cho, Sakyo-ku,
            Kyoto, Kyoto 606-8502, Japan URL :http"; pages: 
            "//www.lif.kyoto-u.ac.jp/e"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      316..1519
                     /label=MpHSP17.8A1 promoter
                     /note="MpHSP17.8A1 promoter"
                     /regulatory_class="promoter"
     protein_bind    1532..1656
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1693..1723
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1777..2433
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2778..3080
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3124..3248)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3280..3532
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3566..3582)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3590..3606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3614..3644)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3659..3680)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    complement(3765..3939)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3999..4787)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4856..5532)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    complement(5727..5751)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6272..7060
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      7309..7897
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8083..8223)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8567..8761)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8830..9900)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10332..10958)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagaccagc cattatagca actatgttaa aagtgacagt tttctctaat
      361 cgggaatctt gtccgtattg aaagttccct gttcagtgac tgagccgctt aataatattt
      421 tcagtttcga gtacgaggga gagattgaac aaattttggg gtttcagggc taaattctgc
      481 aggggcttta gttttgagtc gggtgaaact ttcctaagtc ttggcaatga actcacattc
      541 gccattgtac ctattaaatg acccaaacat ccttgacaac aaattgacaa gtacgggaac
      601 atgatcttca atatgaaatt taatatgtcc tttatgttgg ctttcctcat caatgattgg
      661 aaactcacat acaaagaaaa gcgtgatata ataaatcact agaatcttct ccgctttcta
      721 agacagtaat gatggatcca cgtgagtgtg aaacatcaat atcaacatcg gtaggagtag
      781 agaagtttct aaatcagaaa aagtgaatag acaagacaac ttactccttg aacgagaata
      841 ttcctcgaga aagagggaaa agagtaaaaa gagtaaaaag ccatggtaca gaatagccca
      901 ttccatggtt attgcagtca aaccgctacg ccagcaaaaa cggaacgaaa ctaaacccgt
      961 tccacaaatt gcgaaagatg gaatgtattc gccatgtata tttcgagatt attatgagac
     1021 ttccggtccc gggaagatgg cctgggttca ccgggatcag ccgaattccg acgtgatgga
     1081 gcacggccat gcatacttct agagaagtcg gccaccgtga aattcgacga cgagatggta
     1141 ctagaatcac cgggacagta cacacctcgt tcatggcctc gagcattcga tgtcgctctc
     1201 acctgcacag cctcgaggta tcgagagggt tctaggatgg accagcgatg tctcgaggaa
     1261 tttagaaaga tttcatgcgc tcgttgtgtg agagccaagg aagcacatta tggggcctat
     1321 aagtaccacg caagggcctg gggttcccat caatcaaatc agtgttcaga cgatttcctc
     1381 aagcgatcat tgcaccgtga tcagtgaagc acagacttca agcttttggc ttgcctactt
     1441 ccacgttccc atccgagatc gacaccgaat tgttgagttc tgtctgatct tcctgcagtc
     1501 gagagcgaag ttccaccatc tagagttatc aacaagtttg tacaaaaaag ctgaacgaga
     1561 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     1621 ataatactgt aaaacacaac atatccagtc actatggcgg cccctcgagg ggccgcatta
     1681 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     1741 gatccggcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1801 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1861 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1921 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1981 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     2041 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     2101 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     2161 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     2221 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2281 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2341 attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2401 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
     2461 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     2521 atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag
     2581 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     2641 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     2701 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     2761 gaacagggac tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     2821 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     2881 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     2941 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     3001 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     3061 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     3121 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     3181 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     3241 aaagtggttg ataacagcgc ttagagctcg aatttccccg atcgttcaaa catttggcaa
     3301 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     3361 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     3421 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     3481 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     3541 gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3601 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3661 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctgaatt caacacattg
     3721 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
     3781 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
     3841 acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt
     3901 cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgactc
     3961 tagctagagg atcgatccga accccagagt cccgctcaga agaactcgtc aagaaggcga
     4021 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     4081 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
     4141 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
     4201 atgatattcg gcaagcaggc atcgccatgt gtcacgacga gatcctcgcc gtcgggcatg
     4261 cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
     4321 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
     4381 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
     4441 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc
     4501 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
     4561 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg gagttcattc
     4621 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
     4681 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
     4741 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatccc catggtcgat
     4801 cgacagatct gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc
     4861 gtgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt
     4921 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
     4981 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
     5041 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
     5101 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
     5161 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
     5221 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
     5281 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
     5341 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
     5401 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
     5461 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
     5521 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
     5581 acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgaatt
     5641 cagaaataaa ttcagcctaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg
     5701 ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc
     5761 caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca
     5821 gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg
     5881 atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga
     5941 gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg
     6001 aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc
     6061 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
     6121 catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca
     6181 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     6241 gggcagtcgc cctaaaacaa agttaaacat catgggggaa gcggtgatcg ccgaagtatc
     6301 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     6361 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     6421 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     6481 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     6541 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     6601 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     6661 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     6721 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     6781 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     6841 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     6901 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     6961 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     7021 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag ctagaaattc
     7081 gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca
     7141 taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata
     7201 ataagcccta cacaaattgg gagatatatc atgcatgacc aaaatccctt aacgtgagtt
     7261 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7321 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7381 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7441 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7501 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7561 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7621 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7681 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7741 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7801 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7861 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7921 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7981 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8041 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     8101 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     8161 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     8221 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     8281 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     8341 tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga
     8401 cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg
     8461 gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag
     8521 ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag
     8581 gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt
     8641 ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc
     8701 aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac
     8761 aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc
     8821 cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag
     8881 gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc
     8941 gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc
     9001 gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg
     9061 gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac
     9121 gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat
     9181 gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc
     9241 ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg
     9301 cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc
     9361 tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc
     9421 tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac
     9481 caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc
     9541 gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa
     9601 aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac
     9661 gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc
     9721 cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg
     9781 gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac
     9841 caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac
     9901 gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac aacccagccg
     9961 cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct
    10021 ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat
    10081 tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt
    10141 cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa
    10201 gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct
    10261 gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc
    10321 tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca
    10381 gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag
    10441 ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg
    10501 gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt
    10561 agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc
    10621 catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg
    10681 gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg
    10741 atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga
    10801 tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga
    10861 acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct
    10921 gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt
    10981 tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata
    11041 cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg
    11101 ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt
    11161 gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa
    11221 tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc
    11281 attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg
    11341 ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga
    11401 gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag
    11461 ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc
    11521 tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa
    11581 ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc
    11641 ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc
    11701 cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc
    11761 agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat
    11821 cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa
    11881 gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt
    11941 catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc
    12001 gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg
    12061 cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca
    12121 gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca
    12181 tacaaatgga cgaacggata aacct
//
